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Production Associate I - DNA Subcloning

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Eurofins USA

2021-12-03 07:41:50

Job location Bothell, Washington, United States

Job type: fulltime

Job industry: Science & Technology

Job description

Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins works with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate.

Eurofins is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. Eurofins is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. The Group also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products.

In over just 30 years, Eurofins has grown from one laboratory in Nantes, France to 55,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 50 countries. Eurofins offers a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products.

In 2020, Eurofins generated total revenues of EUR € 5.4 billion, and has been among the best performing stocks in Europe over the past 20 years.

Job Description

Since 1999, Eurofins Genomics Blue Heron(Blue Heron Biotech) has been a pioneer in the gene synthesis industry and has delivered tens of millions of base pairs of perfectly accurate genes to thousands of customers worldwide using its proprietary GeneMaker® multi-technology platform. We are 100% committed to serving our customers with high quality, strict confidentiality standards, and rapid delivery of synthesized genes. In March 2019 Blue Heron was acquired by Eurofins Scientific and became a partner of the Eurofins Genomics US division. Blue Heron Biotech is searching for Production Associates to join our team.

The Production Associate is responsible for performing all activities associated with special cloning (approx. 75%) and performing down/upstream protocols for the company's high-throughput gene synthesis process (approx. 25%).

Position Responsibilities/Accountabilities:

  • Clone DNA into expression cloning vectors.
  • Carry out gene cloning steps, including strategy review, enzymatic DNA restriction and modification, PCR primer design and PCR, gel electrophoresis, band isolation and DNA purification, and ligation using standard and proprietary methods.
  • Track and monitor cloning results and follow-up as needed with sequencing, restriction or PCR analysis.
  • Log-in and store DNA cloning vectors and restriction enzymes, assist with maintaining collections of cloning vector DNA, restriction enzymes and associated documents.
  • Work within a multi-disciplinary team; meet goals for accuracy, production deadlines and throughput utilizing the company's proprietary gene-synthesis platform and related tools.
  • Perform production protocols according to established company operating procedures and assist with general production support as needed.
Qualifications

The ideal candidate is self-motivated, possesses outstanding organizational skills, and the demonstrated ability to perform as an effective team member.

The successful candidate will have a strong background in molecular biology with hands-on knowledge of the uses and properties of DNA and enzymes, PCR and thermocyclers, gel electrophoresis, and photo documentation. Experience utilizing and manipulating data via databases is preferred. Good computer skills, precise attention to detail, record keeping, and adherence to protocols is required. Experiences with liquid handling robots or complex laboratory equipment is desirable.

This 8 h position will require weekend work (Sunday to Thursday, 8 AM to 4:30 PM) or (Tuesday to Saturday 9 am to 5:30 pm).

Job Specifications:

Education:

Associate/BA/BS

Classification

Non-exempt (hourly)

Typical Experience:

0-2 years

Field of Expertise:

Molecular Biology, DNA synthesis, Chemistry, Automation

Supervision:

Minimal direct supervision when trained

Equipment Used:

Thermocyclers, centrifuges, gel electrophoresis equipment, power supplies, liquid handling robots

Software Used:

Windows OS, MS Office, Blue Heron Explorer

Additional Information

All your information will be kept confidential according to EEO guidelines.

Eurofins is a M/F, Disabled, and Veteran Equal Employment Opportunity and Affirmative Action employer.

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