Senior Research Associate, Protein Production Group

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Job description

Role Description:

The Protein Production Group at Jounce Therapeutics is hiring! The successful candidate will play an important role in the discovery and development of novel biotherapeutics for immuno-oncology. They will be responsible for the production and purification of antibodies and other proteins for use in R&D that will enable the development of groundbreaking therapies. The successful candidate will be expected to participate in experimental design, conduct experiments, optimize throughput, maintain detailed records, and participate in cross-functional collaborations. This is an exciting growth opportunity to be part of a collaborative and fun team within the fast-paced and dynamic field of immuno-oncology.

Responsibilities:

• Perform transient protein production in mammalian cell lines;
• Conduct protein purification steps including affinity chromatography, ion-exchange chromatography and size-exclusion chromatography;
• Perform protein characterization methods including SE-HPLC, SDS-PAGE, endotoxin assays and western blots;
• Employ liquid handling robots in the protein production process at smaller scales;
• Utilize automated platforms to generate transfection-grade plasmid DNA; and
• Ensure thorough documentation and cross-functional communication.

Qualifications:

• B.S. or M.S. in biochemistry, bioengineering, chemical engineering or a related discipline;
• Demonstrated ability to master scientific techniques such as:
• Mammalian cell culture and aseptic techniques;
• AKTA purification systems for protein purification (affinity, IEX, SEC);
• Protein analysis (SEC-HPLC, SDS-PAGE, endotoxin assays, Western Blots);
• Excellent attention to detail, strong documentation skills, drive to work in a fast-paced environment, and ability to communicate effectively (both written and verbal);
• Ability to work with LIMS and biological registration systems as well as electronic lab notebook software;
• Experience with bacterial culture and plasmid purification is a plus; and
• Experience with high-throughput cell culture expression and protein purification techniques is a plus.

Company Overview

Jounce Therapeutics, Inc. is a clinical-stage immunotherapy company dedicated to transforming the treatment of cancer by developing therapies that enable the immune system to attack tumors and provide long-lasting benefits to patients through a biomarker-driven approach. Jounce currently has multiple development stage programs ongoing while simultaneously advancing additional early-stage assets from its robust discovery engine based on its Translational Science Platform. Jounce's highest priority program, JTX-8064, is a LILRB2 (ILT4) receptor antagonist shown to reprogram immune-suppressive tumor associated macrophages to an anti-tumor state in preclinical studies. A Phase 1 clinical trial, named INNATE, for JTX-8064 as a monotherapy and in combination with JTX-4014, Jounce's internal PD-1 inhibitor, or pembrolizumab is currently enrolling patients with advanced solid tumors. Jounce's most advanced product candidate, vopratelimab, is a monoclonal antibody that binds to and activates ICOS, and is currently being studied in the SELECT Phase 2 trial. JTX-4014 is a PD-1 inhibitor intended for combination use in the INNATE and SELECT trials and with Jounce's broader pipeline. Additionally, Jounce exclusively licensed worldwide rights to JTX-1811, a monoclonal antibody targeting CCR8 and designed to selectively deplete T regulatory cells in the tumor microenvironment, to Gilead Sciences, Inc. For more information, please visit .

Diversity and Inclusion Statement

Jounce strives to create a workplace that reflects the diversity of cancer patients, and where all feel included and valued because of their differences. Actively including people who add to our culture and enhance our values leads to better decisions and outcomes for those we serve -patients, all patients. Beyond our walls, our focus is on addressing inequities in opportunities for STEM education, careers opportunities in Biotech, and access to cancer care.

Equal Opportunity Employer

Jounce Therapeutics is an equal opportunity employer and does not discriminate in its employment decisions on the basis of race, color, national origin, age, physical or mental disability, marital status, religion, creed, sex, or political beliefs. We offer a competitive salary and benefits package. All applicants should be legally entitled to work for any employer in the U.S.