Scientist I/II, Molecular and Cellular Biology

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Job description

About AbbVie
AbbVie's mission is to discover and deliver innovative medicines that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas: immunology, oncology, neuroscience, eye care, virology, women's health and gastroenterology, in addition to products and services across its Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at . on Twitter , Facebook , Instagram , YouTube and LinkedIn .

AbbVie Immunology is expanding its early discovery team in Cambridge, Massachusetts. The early discovery team in Cambridge is focused on identifying new targets for the treatment of autoimmune and chronic inflammatory diseases through computational analysis of human disease tissue derived datasets, and through validating targets using in vitro and in vivo experimental approaches to make a remarkable impact on patient lives. We are seeking an enthusiastic, skilled, and dedicated individual to join a team that validates targets using genetic tools. This position requires passion, attention to detail, prior hands-on experience, and teamwork.

Responsibilities:

• Performs molecular and cellular biology laboratory procedures following protocols, including mammalian cell culture, bacterial culture, nucleic acid and protein extraction, isolation, and purification, DNA sequencing, primer design, PCR, qRT-PCR, gel electrophoresis, Western blot, lentivirus generation, transfection, transduction, electroporation, and single cell cloning.
• Monitors and operates specialized equipment such as fluorescence activated cell sorters, thermocyclers, real-time PCR instruments, cell counters, and microplate readers.
• Implement in vitro and support in vivo CRISPR tools and platforms, including CRISPRx, CRISPRa, CRISPRi.
• Support target biology validation using genetic tools, including gene overexpression via ORF; CRISPR-based technologies; RNAi; mutagenesis; validation of genetic manipulations.
• Develop and implement assays that monitor cellular processes.
• Maintain accurate laboratory records and data.
• Analyze data and present work in internal meetings.

Qualifications

Qualifications:

Position will be hired based on level of experience

• Bachelor's or Master's in molecular biology, immunology or other biological sciences with 5-7+ (BS) or 2-5+ (MS) years of experience
• Solid understanding of genetic tools such as CRISPR and RNAi-based technologies
• Skillful in standard molecular and cellular biology techniques, such as mammalian cell and bacterial culture, PCR, and gel electrophoresis; experience in genomic DNA isolation and validation of genomic edits, nucleofection using the Lonza 96 shuttle system, qPCR, and lentivirus production is a plus
• Hands-on experience with immunoassays including FACS, ELISA, WB is preferred
• Experience with in vivo animal models, especially immunological models, is a plus
• Bioinformatics experience is a plus
• Excellent communication skills
• Ability to collaborate with peers and their teams

Key AbbVie Competencies:

• Builds strong relationships with peers and cross functionally with partners outside of the team to enable high performance
• Learns fast, grasps the "essence" and can change course quickly where indicated
• Raises the bar and is never satisfied with the status quo
• Creates a learning environment, open to suggestions and experimentation for improvement
• Embraces the ideas of others and nurtures innovation to make possibilities real

Significant Work Activities
N/A
Travel
Yes, 5 % of the Time
Job Type
Experienced
Schedule
Full-time
Job Level Code
IC
Equal Employment Opportunity
At AbbVie, we value bringing together individuals from diverse backgrounds to develop new and innovative solutions for patients. As an equal opportunity employer we do not discriminate on the basis of race, color, religion, national origin, age, sex (including pregnancy), physical or mental disability, medical condition, genetic information gender identity or expression, sexual orientation, marital status, protected veteran status, or any other legally protected characteristic.